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es 2 cells  (ATCC)


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    ATCC es 2 cells
    Es 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/es 2 cells/product/ATCC
    Average 97 stars, based on 1304 article reviews
    es 2 cells - by Bioz Stars, 2026-03
    97/100 stars

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    (A) Basal protein expression of ES2 PR-B+ KO-BRCA1 cell models. Top: Western blot for BRCA1, PR. HDAC2 = loading control. Cell line HCC1937 = negative control for BRCA1. Values under blots represent normalized densitometry units (NDU) for the protein of interest/respective loading control. Graph: NDU for BRCA1 (n=3) shown as mean ± SD, with significance shown as ** p ≤ 0.0030, **** p < 0.0001. (B) Basal transcriptional regulation of BRCA1 mRNA. TBP (TATA-box binding protein) = housekeeping gene. Graph: mean ± SD, **** p< 0.0001. (C) Top Blot: Western blot of BRCA1. Bottom Blot: Western blot for PR, and phosphorylated PR at serine sites (Ser294, Ser345, Ser190, & Ser81) following treatment with vehicle (ethanol) or 10nM R5020 for 1 hour. GAPDH = loading control. Values under blots represent NDU for the protein of interest/respective loading control. Graph: NDU for S294 (top) and S81 (bottom). (D) Transcriptional regulation of PR, FOXO1, and p21 mRNA following treatment with vehicle (ethanol) or 10nM R5020 for 6 hours. TBP = housekeeping gene. Graph represents the mean ± SD, **** p < 0.0001.

    Journal: bioRxiv

    Article Title: Depletion of BRCA1 Potentiates Progestin-Induced Cytoskeletal Changes in an Ovarian Cancer Cell Model

    doi: 10.64898/2026.01.02.697409

    Figure Lengend Snippet: (A) Basal protein expression of ES2 PR-B+ KO-BRCA1 cell models. Top: Western blot for BRCA1, PR. HDAC2 = loading control. Cell line HCC1937 = negative control for BRCA1. Values under blots represent normalized densitometry units (NDU) for the protein of interest/respective loading control. Graph: NDU for BRCA1 (n=3) shown as mean ± SD, with significance shown as ** p ≤ 0.0030, **** p < 0.0001. (B) Basal transcriptional regulation of BRCA1 mRNA. TBP (TATA-box binding protein) = housekeeping gene. Graph: mean ± SD, **** p< 0.0001. (C) Top Blot: Western blot of BRCA1. Bottom Blot: Western blot for PR, and phosphorylated PR at serine sites (Ser294, Ser345, Ser190, & Ser81) following treatment with vehicle (ethanol) or 10nM R5020 for 1 hour. GAPDH = loading control. Values under blots represent NDU for the protein of interest/respective loading control. Graph: NDU for S294 (top) and S81 (bottom). (D) Transcriptional regulation of PR, FOXO1, and p21 mRNA following treatment with vehicle (ethanol) or 10nM R5020 for 6 hours. TBP = housekeeping gene. Graph represents the mean ± SD, **** p < 0.0001.

    Article Snippet: ES2 cells were cultured in McCoy’s 5A medium (ATCC, 30-2007) supplemented with 10% charcoal stripped fetal bovine serum (i.e., DCC; Corning, 35072CV), 1% penicillin-streptomycin (i.e., P/S; Gibco, 15070063), and 0.5mg/ml G418 Sulfate (Corning, 61234RG).

    Techniques: Expressing, Western Blot, Control, Negative Control, Binding Assay